rLys-C, Mass Spec Grade from Promega

Product Specs
ItemrLys-C, Mass Spec Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV1671
Quantity15microg
TypeEnzyme

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Loss of CorA, the primary magnesium transporter of Salmonella, is alleviated by MgtA and PhoP-dependent compensatory mechanismsPLOS ONESeptember 15, 2023Selma Metaane, Véronique Monteil, Thibaut Douché, Quentin Giai Gianetto, Mariette Matondo, Corinne Maufrais, Françoise Noreliodoacetamide (Sigma ‐ I114) for 1H in the dark. Proteins were first digested with 1 μg rLys-C (Promega ‐ V1671) for 3H at 25°C and a second digestion was performed with 1 μg Sequencing Grade Modified Trypsin (Promega

(2) Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesionsNature metabolismAugust 1, 2023Tai YH, Engels D, Locatelli G, Emmanouilidis I, Fecher C, Theodorou D, Müller SA, Licht-Mayer S, Kreutzfeldt M, Wagner I, et al.four washing steps with 200 µl 80% (v/v) ethanol. Proteins were digested with 0.25 µg LysC (Promega, V1671) at 37 °C for 3 h followed by a second digestion step with 0.25 µg trypsin (Promega, V5111) for 16 h

(3) Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 ReceptorbioRxivApril 26, 2023Peng Zhao, Jeremy L. Praissman, Oliver C. Grant, Yongfei Cai, Tianshu Xiao, Katelyn E. Rosenbalm, Kazuhiro Aoki, Benjamin P. Kellman, Robert Bridger, Dan H. Barouch, et al.Aldrich,Cat#43815 Iodoacetamide,Sigma Aldrich,Cat#I1149 Trypsin,Promega,Cat#V5111 Lys-C,Promega,Cat#V1671 Arg-C,Promega,Cat#V1881 Glu-C,Promega,Cat#V1651 Asp-N,Promega,Cat#VA1160 Endoglycosidase H,Promega,Cat#V4871 PNGaseF,Promega,Cat#V4831 Chymotrypsin,Athens Research

(4) Analysis of the Proteomic Profile in Serum of Irradiated Nonhuman Primates Treated with Ex-Rad, a Radiation Medical CountermeasureJournal of Proteome ResearchApril 7, 2023Alana D. Carpenter, Yaoxiang Li, Brianna L. Janocha, Stephen Y. Wise, Oluseyi O. Fatanmi, Manoj Maniar, Amrita K. Cheema, Vijay K. Singhfinal concentration of 15 mM for 30 min at 37 °C in a dark environment with Lys-C (Promega, Cat. # V1671) at 1:100 (w/w) enzyme-to-protein ratio for 3 h at 37 °C. Trypsin Gold (Promega, Cat. # V5280) was added

(5) Multi-Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal ProteinsMolecular & cellular proteomics : MCPMarch 1, 2023Akter F, Bonini S, Ponnaiyan S, Kögler-Mohrbacher B, Bleibaum F, Damme M, Renard BY, Winter D.quenched by addition of 5 mM DTT, samples were diluted to 4 M urea with 0.1 M TEAB, rLys-C (Catalog no. V1671, Promega) added at an enzyme to protein ratio of 1 to 100 (w/w), and digestion was performed at 37 °C

(6) Functional genomics of RAP proteins and their role in mitoribosome regulation in Plasmodium falciparumNature CommunicationsDecember 1, 2022Thomas Hollin et al.proteins were urea-denatured, reduced, alkylated, and digested with endoproteinase Lys-C (Promega, V1671) followed by modified trypsin (Promega, V5111)71. Peptide mixtures were loaded onto 100 μm fused silica

(7) Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesisScience AdvancesNovember 18, 2022Yi Sun, Zujie Yao, Yiting Ye, Jun Fang, Honglin Chen, Yuping Lyu, William Broad, Marjorie Fournier, Genyun Chen, Yonghong Hu, et al.centrifuged at 14,000g for 15 min and then washed twice using 6 M urea in 25 mM AB. LysC protease (Promega, V1671; 200 ng in 50 μl of 6 M urea and 25 mM AB) was added to each filter before incubation at 37°C for 4

(8) Prior Signal Acquisition Software Versions for Orbitrap Underestimate Low Isobaric Mass Tag Intensities, Without Detriment to Differential Abundance ExperimentsACS Measurement Science AuJune 15, 2022Tom S. Smith, Anna Andrejeva, Josie Christopher, Oliver M. Crook, Mohamed Elzek, Kathryn S. Lilleywere digested in a two-step digestion process with the 100:1 protein/protease ratio of Lys-C (Promega; V1671) at 37 °C for 4 h, followed by 100:1 trypsin digestion (Promega; V5111) at 37 °C overnight. Peptide

(9) Three-dimensional feature matching improves coverage for single-cell proteomics based on ion mobility filteringCell SystemsMay 18, 2022Jongmin Woo et al.Scientific,A39271 "Formic acid, LC-MS grade",Thermo Fisher Scientific,28905 "Lys-C, Mass Spectrometry Grade",Promega,V1671 "Trypsin, Mass Spectrometry Grade",Promega,V5280 n-Dodecyl β-D-maltoside (DDM),Sigma Aldrich,D4641–1G 10x

(10) EFhd2 brain interactome reveals its association with different cellular and molecular processesJournal of neurochemistryDecember 1, 2021Soliman AS, Umstead A, Grabinski T, Kanaan NM, Lee A, Ryan J, Lamp J, Vega IE.in the digestion buffer (25 mM AMBIC/50% acetonitrile (ACN)), and rLys-C (500 ng Promega, cat. no. V1671) were added to each sample and incubated at 37°C for 1.5 h. Afterwards, 1 μg trypsin (Promega, cat.

(11) Phosphorylation in the Charged Linker Modulates Interactions and Secretion of Hsp90βCellsJuly 5, 2021Weidenauer L, Quadroni M.in 100 μL 50 mM ammonium bicarbonate with 0.5 μg trypsin (Promega; V5113) and 0.25 μg LysC (Promega; V1671) at 37 °C overnight. Peptides are extracted next day by incubating the gel bands with 100 μL 10% formic

(12) Carbon Catabolite Repression in Filamentous Fungi Is Regulated by Phosphorylation of the Transcription Factor CreAmBioFebruary 23, 2021Leandro José de Assis et al.gain a final volume of 50 μl. Tryptic cleavages were performed using 1 μg rLys C (Promega number [no.] V1671) with incubation for 2 h at 37°C, and subsequently, 2 μg Trypsin Gold (Promega no. V5280) was added

(13) Proteomic Analysis Uncovers Measles Virus Protein C Interaction With p65–iASPP Protein ComplexMolecular & Cellular ProteomicsJanuary 27, 2021Alice Meignié et al.(#I114, Sigma-Aldrich) for 1 h at room temperature in the dark. Samples were digested with rLys-C (#V1671, Promega) ratio 50:1 (protein:rLysC) for 3 h/37 °C and then digested with Sequencing Grade Modified Trypsin

(14) CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studiesPLOS ONEJanuary 11, 2021Yanouk Epelboin et al.iodoacetamide (Sigma—I114) for 30 min at 26˚C in dark. Proteins were digested with 1 μg rLys-C (Promega—V1671) for 3h30 at 30˚C. Samples were then diluted at 1:8 and 0.625 μg of Sequencing Grade Modified Trypsin

(15) Glycomics-informed glycoproteomic analysis of site-specific glycosylation for SARS-CoV-2 spike proteinSTAR ProtocolsDecember 30, 2020Katelyn E. Rosenbalm, Michael Tiemeyer, Lance Wells, Kazuhiro Aoki, Peng Zhaoreagent",Sigma-Aldrich,320331 Dithiothreitol,Sigma-Aldrich,43815 Iodoacetamide,Sigma-Aldrich,I1149 Trypsin,Promega,V5111 Lys-C,Promega,V1671 Arg-C,Promega,V1881 Glu-C,Promega,V1651 Asp-N,Promega,VA1160 Endoglycosidase H,Promega,V4871 PNGaseF,Promega,V4831 Chymotrypsin,Athens Research

(16) Yeast Nuak1 phosphorylates histone H3 threonine 11 in low glucose stress by the cooperation of AMPK and CK2 signalingeLifeDecember 29, 2020Seunghee Oh, Jaehyoun Lee, Selene K Swanson, Laurence Florens, Michael P Washburn, Jerry L Workman,New England Biolabs,Cat# P0753, "Peptide, recombinant protein","rLys-C, Mass Spec Grade",Promega,Cat# V1671, "Peptide, recombinant protein",Sequencing Grade Modified Trypsin,Promega,Cat# V5111, Commercial assay

(17) An optimized quantitative proteomics method establishes the cell type‐resolved mouse brain secretomeThe EMBO JournalOctober 15, 2020Johanna Tüshaus et al.Aldrich,Cat#30970 Ammonium bicarbonate,Sigma Aldrich,Cat#5.33005 Trypsin,Promega,Cat#V5111 LysC,Promega,Cat#V1671 "ReproSil‐Pur 120 C18‐AQ, 1.9 μm",Dr. Maisch GmbH,Cat#r119.aq. 30 cm Uncoated SilicaTip Emitters,New

(18) Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 ReceptorCell Host & MicrobeOctober 1, 2020Peng Zhao, Jeremy L. Praissman, Oliver C. Grant, Yongfei Cai, Tianshu Xiao, Katelyn E. Rosenbalm, Kazuhiro Aoki, Benjamin P. Kellman, Robert Bridger, Dan H. Barouch, et al.Aldrich,Cat#43815 Iodoacetamide,Sigma Aldrich,Cat#I1149 Trypsin,Promega,Cat#V5111 Lys-C,Promega,Cat#V1671 Arg-C,Promega,Cat#V1881 Glu-C,Promega,Cat#V1651 Asp-N,Promega,Cat#VA1160 Endoglycosidase H,Promega,Cat#V4871 PNGaseF,Promega,Cat#V4831 Chymotrypsin,Athens Research

(19) Integrative Modeling of a Sin3/HDAC Complex Sub-structureCell ReportsApril 14, 2020Charles A.S. Banks, Ying Zhang, Sayem Miah, Yan Hao, Mark K. Adams, Zhihui Wen, Janet L. Thornton, Laurence Florens, Michael P. WashburnHigh Quality (Bioprocessing grade),ArcticZymes,Cat# 70920-202 "rLys-C, Mass Spec Grade",Promega,Cat# V1671 Sequencing Grade Modified Trypsin,Promega,Cat# V5117 2-Chloroacetamide,Sigma,Cat# C0267 Bestatin

(20) Single-cell proteomics reveals changes in expression during hair-cell developmenteLifeNovember 4, 2019Zhu Y, Scheibinger M, Ellwanger DC, Krey JF, Choi D, Kelly RT, Heller S, Barr-Gillespie PG.(w/v) "Chemical compound, drug",trypsin,Promega,Cat# V5280,10 ng/µl "Chemical compound, drug",Lys-C,Promega,Cat# V1671,10 ng/µl "Chemical compound, drug",DAPI,Thermo Fisher,Cat# D1306,1 µg/ml "Chemical compound, drug",Alexa

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